This study examines whether gender differences in teacher-assigned grades vary systematically across subjects. Using data from German secondary schools, I compare teacher-assigned grades with anonymously graded standardized test scores within a difference-in-differences framework. Across six core subjects spanning the humanities and STEM, I find systematic grading differentials favoring female students. The estimated differential is largest in German and Math and substantially smaller in English and the sciences, indicating considerable cross-subject heterogeneity. The differential also varies markedly by school track and teacher experience, with systematically larger estimates in the academic track and particularly pronounced differentials among novice teachers.